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Product Name
PIWIL1 antibody
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Description
PIWIL1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human liver cancer tissue, human prostate cancer tissue. Positive WB detected in human testis tissue, mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 85-95 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
HIWI antibody; MIWI antibody; PIWI antibody; piwi like 1 (Drosophila) antibody; Piwi like protein 1 antibody; PIWIL1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIWIL1 recombinant protein (Accession Number: XM_011539004). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113841(PIWIL1 Antibody) at dilution of 1:600
IP Result of anti-PIWIL1 (IP:Catalog No:113841, 4ug; Detection:Catalog No:113841 1:500) with mouse testis tissue lysate 2400ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113841(PIWIL1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:113841(PIWIL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PIWIL1 represents piwi-like 1 (Drosophila)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"