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Product Name
PITX2 Polyclonal Antibody
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Description
Polyclonal antibody to PITX2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PITX2 antibody; ARP1 antibody; ASGD4 antibody; Brx1 antibody; IDG2 antibody; IGDS antibody; IGDS2 antibody; IHG2 antibody; IRID2 antibody; Otlx2 antibody; PTX2 antibody; RGS antibody; RIEG antibody; RIEG1 antibody; RS antibody; pituitary homeobox 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PITX2 (NP_000316.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - PITX2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PITX2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform PTX2C is involved in left-right asymmetry the developing embryo (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"