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Product Name
PIR antibody
- Documents
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Description
PIR Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PIR antibody; Pirin antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIR recombinant protein (Accession Number: NM_003662). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113833(PIR antibody) at dilution of 1:500
IP Result of anti-PIR (IP:Catalog No:113833, 4ug; Detection:Catalog No:113833 1:300) with HeLa cells lysate 1200ug.
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Background
Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"