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Product Name
PIGS antibody
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Description
PIGS Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, HepG2 cells, mouse liver tissue, PC-3 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp686K20216 antibody; FLJ45226 antibody; PIGS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIGS recombinant protein (Accession Number: BC001319). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113897(PIGS Antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells using Catalog No:113897(PIGS Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PIGS, also named as GPI transamidase component PIG-S or PRO4316, is a 555 amino acid protein, which belongs to the PIGS family. PIGS localizes in the endoplasmic reticulum membrane. PIGS is a component of the GPI transamidase complex. It is essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"