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Product Name
PIGO antibody
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Description
PIGO Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, COLO 320 cells, mouse spleen tissue. Positive IHC detected in human liver tissue, human kidney tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 74kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434M222 antibody; FLJ00135 antibody; PIG O antibody; PIGO antibody; RP11 182N22.4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIGO recombinant protein (Accession Number: BC013987). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113895(PIGO antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:113895(PIGO antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:113895(PIGO antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PIGO antibody Catalog No:113895 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PIGO represents phosphatidylinositol glycan anchor biosynthesis, class O
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"