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Piezo1 antibody

Cat.#: 113887

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Special Price 239.0 USD

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Product Information

  • Product Name
    Piezo1 antibody
  • Documents
  • Description
    Piezo1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, COLO 320 cells, HeLa cells, human brain tissue, HUVEC cells. Observed molecular weight by Western-blot: 230-280 kDa
  • Tested applications
    ELISA, WB
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    FAM38A antibody; KIAA0233 antibody; Mib antibody; PIEZO1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Piezo1 recombinant protein (Accession Number: BC008073). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113887(FAM38A antibody) at dilution of 1:400

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113887(FAM38A antibody) at dilution of 1:400

  • Background
    Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as PIEZO1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Fam38A also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding PIEZO1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers.
  • References
    • Eisenhoffer GT, Loftus PD, Yoshigi M. Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia. Nature. 484(7395):546-9. 2012.
    • Li J, Hou B, Tumova S. Piezo1 integration of vascular architecture with physiological force. Nature. 515(7526):279-82. 2014.
    • Fotiou E, Martin-Almedina S, Simpson MA. Novel mutations in PIEZO1 cause an autosomal recessive generalized lymphatic dysplasia with non-immune hydrops fetalis. Nature communications. 6:8085. 2015.
    • Andolfo I, Alper SL, De Franceschi L. Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1. Blood. 121(19):3925-35, S1-12. 2013.
    • Miyamoto T, Mochizuki T, Nakagomi H. Functional role for Piezo1 in stretch-evoked Ca²⁺ influx and ATP release in urothelial cell cultures. The Journal of biological chemistry. 289(23):16565-75. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"