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Product Name
PIBF1 antibody
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Description
PIBF1 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells, MCF7 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C13orf24 antibody; CEP90 antibody; KIAA1008 antibody; PIBF antibody; PIBF1 antibody; RP11 505F3.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIBF1 recombinant protein (Accession Number: NM_006346). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113885(PIBF1 Antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using PIBF1 antibody Catalog No:113885 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PIBF1 (IP:Catalog No:113885, 4ug; Detection:Catalog No:113885 1:500) with HEK-293 cells lysate 1120ug.
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Background
PIBF1 represents progesterone immunomodulatory binding factor 1
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References
- Kodani A, Yu TW, Johnson JR. Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication. eLife. 4:. 2015.
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