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Product Name
PIAS4 antibody
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Description
PIAS4 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HL-60 cells, mouse ovary tissue, mouse pancreas tissue. Observed molecular weight by Western-blot: 57-70kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
E3 SUMO protein ligase PIAS4 antibody; FLJ12419 antibody; PIAS gamma antibody; PIAS4 antibody; Piasg antibody; PIASY antibody; ZMIZ6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIAS4 recombinant protein (Accession Number: NM_015897). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113884(PIAS4 antibody) at dilution of 1:300
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Background
PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).
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References
- Torikoshi K, Abe H, Matsubara T. Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells. PloS one. 7(7):e41186. 2012.
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