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Product Name
PI4KB Polyclonal Antibody
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Description
Polyclonal antibody to PI4KB
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PI4KB antibody; NPIK antibody; PI4K-BETA antibody; PI4K92 antibody; PI4KBETA antibody; PI4KIIIBETA antibody; PIK4CB antibody; phosphatidylinositol 4-kinase beta antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 560-801 of human PI4KB (NP_001185702).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - PI4KB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PI4KB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"