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Product Name
PI4KA antibody
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Description
PI4KA Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human placenta tissue. Positive WB detected in human brain tissue, HeLa cells, mouse brain tissue, NIH-3T3 cells (RNAi). Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 231 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ16556 antibody; PI4 kinase alpha antibody; PI4K ALPHA antibody; pi4K230 antibody; PI4KA antibody; PIK4 antibody; PIK4CA antibody; PtdIns 4 kinase alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PI4KA recombinant protein (Accession Number: BC018120). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
WB result of anti- PI4KA (Catalog No:113878) with NIH-3T3 cells (RNAi).
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113878(PI4KA antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113878(PI4KA antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PI4KA (IP:Catalog No:113878, 5ug; Detection:Catalog No:113878 1:500) with mouse brain tissue lysate 4000ug.
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Background
PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).
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References
- Akimana C, Al-Khodor S, Abu Kwaik Y. Host factors required for modulation of phagosome biogenesis and proliferation of Francisella tularensis within the cytosol. PloS one. 5(6):e11025. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"