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Product Name
PI3K p110(beta) antibody
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Description
PI3K p110(beta) Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, rat liver tissue. Positive IHC detected in human skin tissue, human lung cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp779K1237 antibody; p110 BETA antibody; PI3 Kinase p110 beta antibody; PI3 kinase subunit beta antibody; PI3K antibody; PI3K beta antibody; PI3K p110(beta) antibody; PI3Kbeta antibody; PI3KCB antibody; PIK3C1 antibody; PIK3CB antibody; PtdIns 3 kinase subunit beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_006219). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113873(PIK3CB antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human skin slide using Catalog No:113873(PIK3CB Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human skin slide using Catalog No:113873(PIK3CB Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using PIK3CB antibody Catalog No:113873 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.
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References
- Wu DM, Zhang P, Liu RY. Phosphorylation and changes in the distribution of nucleolin promote tumor metastasis via the PI3K/Akt pathway in colorectal carcinoma. FEBS letters. 588(10):1921-9. 2014.
- Li X, Zou K, Gou J. Effect of baicalin-copper on the induction of apoptosis in human hepatoblastoma cancer HepG2 cells. Medical oncology (Northwood, London, England). 32(3):72. 2015.
- Huang W, Liu J, Feng X. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. Medical oncology (Northwood, London, England). 32(4):115. 2015.
- Liu J, Huang W, Ren C. Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways. Scientific reports. 5:11614. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"