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Product Name
Human PLA2G1B (His Tag) recombinant protein
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Description
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
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Protein name
Phospholipase A2
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Protein short names
PPLA2; PLA2-IB; SPLA2IB; PLA2; PLA2G1B; MGC119834; MGC119835; PLA2A; MGC6679
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Uniprot ID
P04054
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Gene Name
PLA2G1B; PLA2; PLA2A; PPLA2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human PLA2G1B (NP_000919.1) (Met 1-Ser 148) with a C-terminal polyhistidine tag was expressed.
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Protein Species
Human
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Molecular weight
The recombinant human PLA2G1B comprises 144 amino acids after removal of the signal peptide, and has a predicted molecular mass of 16.2 kDa. rh PLA2G1B migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 92 % as determined by SDS-PAGE
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Activity
Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol.
The specific activity is >2,500 pmol/min/μg. -
Validations
Human PLA2G1B / PLA2 Protein (His Tag) SDS-PAGE
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