Product Name

Phospholemman/FXYD1 antibody

Documents

Description

Phospholemman/FXYD1 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human skeletal muscle tissue. Positive IP detected in mouse heart tissue. Positive WB detected in mouse skeletal muscle tissue, human brain tissue, human skeletal muscle tissue, mouse heart tissue, mouse kidney tissue, rat heart tissue, rat skeletal muscle tissue. Observed molecular weight by Western-blot: 10-15 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

FXYD1 antibody; Phospholemman antibody; PLM antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Phospholemman/FXYD1 recombinant protein (Accession Number: NM_001278717). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:2000

IHC: 1:20-1:200

Validations

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:113828(FXYD1 antibody) at dilution of 1:200

Immunohistochemical of paraffin-embedded human heart using Catalog No:113828(FXYD1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human heart using Catalog No:113828(FXYD1 antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-FXYD1 (IP:Catalog No:113828, 4ug; Detection:Catalog No:113828 1:500) with mouse heart tissue lysate 4000ug.

Background

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

References

Heiny JA, Kravtsova VV, Mandel F. The nicotinic acetylcholine receptor and the Na,K-ATPase alpha2 isoform interact to regulate membrane electrogenesis in skeletal muscle. The Journal of biological chemistry. 285(37):28614-26. 2010.
Benziane B, Widegren U, Pirkmajer S, Henriksson J, Stepto NK, Chibalin AV. Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle. American journal of physiology. Endocrinology and metabolism. 301(3):E456-66. 2011.
Boon H, Kostovski E, Pirkmajer S. Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans. American journal of physiology. Endocrinology and metabolism. 302(7):E864-71. 2012.
Chibalin AV, Heiny JA, Benziane B. Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman. PloS one. 7(3):e33719. 2012.
Thomassen M, Murphy RM, Bangsbo J. Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans. The Journal of physiology. 591(6):1523-33. 2013.
Skovgaard C, Christensen PM, Larsen S, Andersen TR, Thomassen M, Bangsbo J. Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners. Journal of applied physiology (Bethesda, Md. : 1985). 117(10):1097-109. 2014.
Hostrup M, Kalsen A, Onslev J. Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men. Journal of applied physiology (Bethesda, Md. : 1985). 119(5):475-86. 2015.
Manoharan P, Radzyukevich TL, Hakim Javadi H. Phospholemman is not required for the acute stimulation of Na+-K+-ATPase α2-activity during skeletal muscle fatigue. American journal of physiology. Cell physiology. 309(12):C813-22. 2015.

Phospholemman/FXYD1 antibody

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