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  • Phospho-TBC1D4-T642 pAb

Phospho-TBC1D4-T642 pAb

Cat.#: 166520

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Product Information

  • Product Name
    Phospho-TBC1D4-T642 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-TBC1D4-T642
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse
  • Alternative names
    TBC1D4 antibody; AS160 antibody; NIDDM5 antibody; TBC1 domain family member 4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around T642 of human TBC1D4 (NP_055647.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-TBC1D4-T642 pAb

    Western blot - Phospho-TBC1D4-T642 pAb

    Western blot analysis of extracts of 293T cells, using Phospho-TBC1D4-T642 antibody at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/ml) for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit .Exposure time: 30s.

  • Background
    May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"