Polyclonal antibody to Phospho-TBC1D4-T642
TBC1D4 antibody; AS160 antibody; NIDDM5 antibody; TBC1 domain family member 4 antibody
Antigen: A synthetic phosphorylated peptide around T642 of human TBC1D4 (NP_055647.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-TBC1D4-T642 pAb
Western blot analysis of extracts of 293T cells, using Phospho-TBC1D4-T642 antibody at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/ml) for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit .Exposure time: 30s.
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
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