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  • Phospho-PEA15-S104 pAb

Phospho-PEA15-S104 pAb

Cat.#: 166518

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-PEA15-S104 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-PEA15-S104
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    PEA15 antibody; HMAT1 antibody; HUMMAT1H antibody; MAT1 antibody; MAT1H antibody; PEA-15 antibody; PED antibody; astrocytic phosphoprotein PEA-15 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-PEA15-S104 pAb

    Western blot - Phospho-PEA15-S104 pAb

    Western blot analysis of extracts of HeLa and C6 cells, using Phospho-PEA15-S104 antibody at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 10s.

  • Background
    Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"