Polyclonal antibody to Phospho-NF2-S518
Human, Mouse, Rat
NF2 antibody; ACN antibody; BANF antibody; SCH antibody; merlin antibody
Antigen: A phospho specific peptide corresponding to residues surrounding S518 of human NF2
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IF 1:100 - 1:200
Western blot - Phospho-NF2-S518 pAb
Western blot analysis of extracts from HUVEC cells, using Phospho-NF2-S518 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunofluorescence - Phospho-NF2-S518 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-NF2-S518 antibody .
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
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