Polyclonal antibody to Phospho-MKNK1-T197/202
Human, Mouse, Rat
MKNK1 antibody; MNK1 antibody; MAP kinase interacting serine/threonine kinase 1 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding T197/202 of human MKNK1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-MKNK1-T197/202 pAb
Western blot analysis of extracts of various cell lines, using Phospho-MKNK1-T197/202 antibody at 1:1000 dilution. HeLa cells were treated by 10% FBS for after serum-starvation overnight or treated by ATP (5mM) at 30u2103 for 1 hour .NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30u2103 for 1 hour .PC-12 cells were treated by 20% FBS for 20 minutes after serum-starvation overnight or treated by ATP (5mM) at 30u2103 for 1 hourSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
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