NovoPro
Cart 2
  • Phospho-LIMK1-T508 pAb
  • Phospho-LIMK1-T508 pAb
  • Phospho-LIMK1-T508 pAb

Phospho-LIMK1-T508 pAb

Cat.#: 166423

Special Price 232.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Phospho-LIMK1-T508 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-LIMK1-T508
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    LIMK1 antibody; LIMK antibody; LIMK-1 antibody; LIM domain kinase 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding T508 of human LIMK1
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:100 - 1:200
  • Validations

    Western blot - Phospho-LIMK1-T508 pAb

    Western blot - Phospho-LIMK1-T508 pAb

    Western blot analysis of extracts from HeLa cells, using Phospho-LIMK1-T508 antibody .

    Immunohistochemistry - Phospho-LIMK1-T508 pAb

    Immunohistochemistry - Phospho-LIMK1-T508 pAb

    Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-LIMK1-T508 antibody .

    Immunofluorescence - Phospho-LIMK1-T508 pAb

    Immunofluorescence - Phospho-LIMK1-T508 pAb

    Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-LIMK1-T508 antibody .

  • Background
    Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"