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  • Phospho-IL10RA-S319/S323 pAb

Phospho-IL10RA-S319/S323 pAb

Cat.#: 166414

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Product Information

  • Product Name
    Phospho-IL10RA-S319/S323 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-IL10RA-S319/S323
  • Tested applications
    WB
  • Species reactivity
    Human
  • Alternative names
    IL10RA antibody; CD210 antibody; CD210a antibody; CDW210A antibody; HIL-10R antibody; IL-10R1 antibody; IL10R antibody; interleukin-10 receptor subunit alpha antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding S319/S323 of human IL10RA
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-IL10RA-S319/S323 pAb

    Western blot - Phospho-IL10RA-S319/S323 pAb

    Western blot analysis of extracts from HeLa cells, using Phospho-IL10RA-S319/S323 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

  • Background
    Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"