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  • Phospho-Histone H4-S1 pAb

Phospho-Histone H4-S1 pAb

Cat.#: 166552

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-Histone H4-S1 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-Histone H4-S1
  • Tested applications
    WB, IHC
  • Species reactivity
    Human
  • Alternative names
    antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding S1 of human Histone H4.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:100 - 1:200
  • Validations

    Western blot - Phospho-Histone H4-S1 pAb

    Western blot - Phospho-Histone H4-S1 pAb

    Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37u2103 for 1 hour or Nocodazole (50 ng/ml) at 37u2103 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 180s.

  • Background
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"