Phospho-Histone H4-S1 pAb
Polyclonal antibody to Phospho-Histone H4-S1
Antigen: A phospho specific peptide corresponding to residues surrounding S1 of human Histone H4.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:100 - 1:200
Western blot - Phospho-Histone H4-S1 pAb
Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37u2103 for 1 hour or Nocodazole (50 ng/ml) at 37u2103 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 180s.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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