Polyclonal antibody to Phospho-EphA3-Y779
EPHA3 antibody; EK4 antibody; ETK antibody; ETK1 antibody; HEK antibody; HEK4 antibody; TYRO4 antibody; EPH receptor A3 antibody
Antigen: A synthetic phosphorylated peptide around Y779 of human EphA3 (NP_005224.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-EphA3-Y779 pAb
Western blot analysis of extracts of 293T and Hela cells, using Phospho-EphA3-Y779 antibody at 1:2000 dilution. 293T cells were treated by Pervanadate (1mM) for 30 minutes after serum-starvation overnight.HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
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