Polyclonal antibody to Phospho-ATR-S428
ATR antibody; FCTCS antibody; FRP1 antibody; MEC1 antibody; SCKL antibody; SCKL1 antibody; ATR serine/threonine kinase antibody
Antigen: A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IF 1:50 - 1:200
Immunofluorescence - Phospho-ATR-S428 pAb
Immunofluorescence analysis of U2OS cells using Phospho-ATR-S428 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
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