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Product Name
PHLPPL antibody
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Description
PHLPPL Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human colon cancer tissue, human colon tissue. Positive IP detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, HEK-293 cells, HeLa cells, HT-1080 cells, mouse brain tissue. Observed molecular weight by Western-blot: 147 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
KIAA0931 antibody; PHLPP like antibody; PHLPP2 antibody; PHLPPL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PHLPPL recombinant protein (Accession Number: NM_015020). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113826(PHLPPL Antibody) at dilution of 1:600
IP Result of anti-PHLPPL (IP:Catalog No:113826, 4ug; Detection:Catalog No:113826 1:600) with NIH/3T3 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:113826(PHLPPL Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:113826(PHLPPL Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
PHLPPL also named as KIAA0931 or PHLPP2 is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.
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