-
Product Name
PHF17 antibody
- Documents
-
Description
PHF17 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 58-61kd ,94-96kd
-
Tested applications
ELISA, WB
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
FLJ22479 antibody; JADE1 antibody; KIAA1807 antibody; PHD finger protein 17 antibody; PHF17 antibody; Protein Jade 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PHF17 recombinant protein (Accession Number: NM_024900). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113809(PHF17 antibody) at dilution of 1:500
-
Background
PHF17 was identified as a protein partner of the von Hippel-Lindau tumor suppressor pVHL. It is one component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It can act as a transcriptional coactivator that promote acetylation of nucleosomal histone H4 by KAT5. In addition, it ubiquitinates both phosphorylated and non-phosphorylated β-catenin and therefore regulates canonical Wnt signaling in both Wnt-off and Wnt-on phases.
-
References
- Borgal L, Habbig S, Hatzold J. The ciliary protein nephrocystin-4 translocates the canonical Wnt regulator Jade-1 to the nucleus to negatively regulate β-catenin signaling. The Journal of biological chemistry. 287(30):25370-80. 2012.
- Borgal L, Rinschen MM, Dafinger C. Casein kinase 1 α phosphorylates the Wnt regulator Jade-1 and modulates its activity. The Journal of biological chemistry. 289(38):26344-56. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"