-
Product Name
PHD3 antibody
- Documents
-
Description
PHD3 Rabbit Polyclonal antibody. Positive WB detected in HT-1080 cells, A375 cells, HEK-293 cells, Jurkat cells. Positive IP detected in A375 cells. Positive IHC detected in human brain tissue, human heart tissue. Observed molecular weight by Western-blot: 28-32k kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human; other species not tested.
-
Alternative names
Egl nine homolog 3 antibody; EGLN3 antibody; FLJ21620 antibody; HIF PH3 antibody; HIF prolyl hydroxylase 3 antibody; HIFPH3 antibody; HPH 1 antibody; HPH 3 antibody; PHD3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PHD3 recombinant protein (Accession Number: NM_022073). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:113805(EGLN3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:113805(EGLN3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113805(EGLN3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-EGLN3 (IP:Catalog No:113805, 4ug; Detection:Catalog No:113805 1:500) with A375 cells lysate 3600ug.
-
Background
EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.
-
References
- Su C, Huang K, Sun L. Overexpression of the HIF hydroxylase PHD3 is a favorable prognosticator for gastric cancer. Medical oncology (Northwood, London, England). 29(4):2710-5. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"