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Product Name
PHC1 Polyclonal Antibody
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Description
Polyclonal antibody to PHC1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PHC1 antibody; EDR1 antibody; HPH1 antibody; MCPH11 antibody; RAE28 antibody; polyhomeotic homolog 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 750-940 of human PHC1 (NP_004417.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - PHC1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PHC1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - PHC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human adenomyosis using PHC1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - PHC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using PHC1 antibody at dilution of 1:100 (40x lens).
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Background
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"