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Product Name
PGM1 antibody
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Description
PGM1 Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in Hepg2 cells, HEK-293 cells. Observed molecular weight by Western-blot: 61kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Glucose phosphomutase 1 antibody; PGM 1 antibody; PGM1 antibody; phosphoglucomutase 1 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of PGM1 recombinant protein (Accession Number: NM_002633). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107467(PGM1 antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells using Catalog No:107467(PGM1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
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