PGC1 alpha Polyclonal Antibody

Cat.#: 161043

Special Price 174.2 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    PGC1 alpha Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to PGC1 alpha
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    PPARGC1A antibody; LEM6 antibody; PGC-1(alpha) antibody; PGC-1alpha antibody; PGC-1v antibody; PGC1 antibody; PGC1A antibody; PPARGC1 antibody; PPARG coactivator 1 alpha antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PGC1 alpha (NP_037393.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - PGC1 alpha Polyclonal Antibody

    Western blot - PGC1 alpha Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using PGC1 alpha antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunofluorescence - PGC1 alpha Polyclonal Antibody

    Immunofluorescence - PGC1 alpha Polyclonal Antibody

    Immunofluorescence analysis of C6 cells using PGC1 alpha antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - PGC1 alpha Polyclonal Antibody

    Immunofluorescence - PGC1 alpha Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using PGC1 alpha antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - PGC1 alpha Polyclonal Antibody

    Immunofluorescence - PGC1 alpha Polyclonal Antibody

    Immunofluorescence analysis of NIH/3T3 cells using PGC1 alpha antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"