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PEX16 antibody

Cat.#: 113728

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Product Information

  • Product Name
    PEX16 antibody
  • Documents
  • Description
    PEX16 Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 38kd
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Peroxin 16 antibody; PEX16 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PEX16 recombinant protein (Accession Number: NM_057174). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113728(PEX16 antibody) at dilution of 1:500

    human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113728(PEX16 antibody) at dilution of 1:500

    Immunofluorescent analysis of HepG2 cells using Catalog No:113728(PEX16 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:113728(PEX16 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).
  • References
    • Yonekawa S, Furuno A, Baba T. Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America. 108(31):12746-51. 2011.
    • Kovacs WJ, Charles KN, Walter KM. Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice. Biochimica et biophysica acta. 1821(6):895-907. 2012.
    • Walter KM, Schönenberger MJ, Trötzmüller M. Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy. Cell metabolism. 20(5):882-97. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"