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Product Name
peroxiredoxin 2 antibody
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Description
peroxiredoxin 2 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IF detected in HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, HepG2 cells, human brain tissue, SMMC-7721 cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NKEF B antibody; NKEFB antibody; peroxiredoxin 2 antibody; PRDX2 antibody; PRP antibody; PRX II antibody; PRX2 antibody; PRXII antibody; TDPX1 antibody; Thioredoxin peroxidase 1 antibody; TSA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of peroxiredoxin 2 recombinant protein (Accession Number: NM_005809). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113721(PRDX2 antibody) at dilution of 1:50 (under 10x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113721(PRDX2 Antibody) at dilution of 1:1000
IP Result of anti-PRDX2 (IP:Catalog No:113721, 4ug; Detection:Catalog No:113721 1:1000) with HEK-293 cells lysate 2800ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:113721(PRDX2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)
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References
- Jaraj SJ, Egevad L. Formalin fixation and immunoreactivity in prostate cancer and benign prostatic tissues. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 118(5):383-8. 2010.
- Wu XY, Fu ZX, Wang XH. Peroxiredoxins in colorectal neoplasms. Histology and histopathology. 25(10):1297-303. 2010.
- Yang S, Luo A, Hao X. Peroxiredoxin 2 inhibits granulosa cell apoptosis during follicle atresia through the NFKB pathway in mice. Biology of reproduction. 84(6):1182-9. 2011.
- Suleman F, Gualeni B, Gregson HJ. A novel form of chondrocyte stress is triggered by a COMP mutation causing pseudoachondroplasia. Human mutation. 33(1):218-31. 2012.
- Su TR, Lin JJ, Chiu CC. Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells. Electrophoresis. 33(7):1139-52. 2012.
- Otani A, Kojima H, Guo C, Oishi A, Yoshimura N. Low-dose-rate, low-dose irradiation delays neurodegeneration in a model of retinitis pigmentosa. The American journal of pathology. 180(1):328-36. 2012.
- Ho KY, Yeh TS, Huang HH. Upregulation of phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in acquired middle ear cholesteatoma growth. International journal of molecular sciences. 14(7):14439-59. 2013.
- Kikuta K, Tochigi N, Saito S. Peroxiredoxin 2 as a chemotherapy responsiveness biomarker candidate in osteosarcoma revealed by proteomics. Proteomics. Clinical applications. 4(5):560-7. 2010.
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