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Product Name
peroxiredoxin 2 antibody
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Description
peroxiredoxin 2 Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 23kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
NKEF B antibody; NKEFB antibody; peroxiredoxin 2 antibody; PRDX2 antibody; PRP antibody; PRX2 antibody; PRXII antibody; TDPX1 antibody; Thioredoxin peroxidase 1 antibody; TSA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005809). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113720(PRDX2 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using PRDX2 antibody Catalog No:113720 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PRDX2, also named as NKEFB, TDPX1, TSA and PRP, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. PRDX2 may play an important role in eliminating peroxides generated during metabolism. It might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The antibody is specific to PRDX2.
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