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Product Name
PER1 antibody
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Description
PER1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in A549 cells, human brain tissue, mouse kidney tissue, mouse liver tissue. Observed molecular weight by Western-blot: 50-55kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hPER antibody; hPER1 antibody; KIAA0482 antibody; PER antibody; PER1 antibody; period homolog 1 (Drosophila) antibody; RIGUI antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PER1 recombinant protein (Accession Number: BC028207). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:113711(PER1 antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using PER1 antibody Catalog No:113711 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143].
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