Pep-RTYM peptide

Pep-RTYM peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-DHVTPDIAYNPRTMY-OH

H-Asp-His-Val-Thr-Pro-Asp-Ile-Ala-Tyr-Asn-Pro-Arg-Thr-Met-Tyr-OH

15

95.2%

C₇₉H₁₁₇N₂₁O₂₅S

1792.95

Synthetic

Powder

Pep-RTYM is a synthetic bioactive peptide derived from <i>Acacia catechu</i>. Pep-RTYM inhibited DENV infection with a half-maximal inhibition concentration value of 7.9 uM. Time-of-addition study demonstrated that Pep-RTYM interacted with DENV particles and inhibited cellular entry. Pep-RTYM at 50 uM significantly reduced DENV production in Vero-kidney epithelial cells about 1000-fold, but it could decrease the virus production in Huh7 hepatocyte cells approximately 40-fold. Binding of Pep-RTYM to DENV particles may prevent virus interaction with cellular receptor and subsequent virus entry. Pep-RTYM interacts with DENV particles and inhibited cell entry, and inhibited DENV replication and production in a dose-dependent manner. This finding suggests a potential role of Pep-RTYM in the development of a novel anti-DENV drug.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Panya, A.; Sawasdee, N.; Songprakhon, P.; Tragoolpua, Y.; Rotarayanont, S.; Choowongkomon, K.; Yenchitsomanus, P.-T. A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry. Viruses 2020, 12, 1267.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.