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Product Name
Pentraxin 3 antibody
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Description
Pentraxin 3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, Jurkat cells. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Pentaxin related protein PTX3 antibody; Pentraxin 3 antibody; Pentraxin related protein PTX3 antibody; PTX3 antibody; TNF alpha induced protein 5 antibody; TNFAIP5 antibody; TSG 14 antibody; TSG14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Pentraxin 3 recombinant protein (Accession Number: NM_002852). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113706(PTX3 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells using Catalog No:113706(PTX3 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PTX3, also named as TNFAIP5 and TSG14, is an acute phase glycoprotein that plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. In WB, there're 2 major bands, an upper signal at 80-86 kDa and a lower signal at 43 kDa, representing PTX3 dimers and monomers, respectively(18223257).
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References
- Chen SX, Xu XE, Wang XQ. Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation. Journal of proteomics. 110:155-71. 2014.
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