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Product Name
PELO antibody
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Description
PELO Rabbit Polyclonal antibody. Positive WB detected in SKOV-3 cells, HEK-293 cells, HeLa cells, mouse cerebellum tissue, mouse thymus tissue, SH-SY5Y cells. Positive IP detected in HEK-293 cells. Positive IF detected in U2OS cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 43-45 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 17 antibody; PELO antibody; pelota homolog (Drosophila) antibody; PRO1770 antibody; Protein pelota homolog antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PELO recombinant protein (Accession Number: NM_015946). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113705(PELO antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113705(PELO antibody) at dilution of 1:100 (under 25x lens)
IF result of Catalog No:113705(PELO antibody) in U2OS cell by Dr. Nancy Kedersha.(cytoplasm and nucleus; Green).
IP Result of anti-PELO (IP:Catalog No:113705, 4ug; Detection:Catalog No:113705 1:500) with HEK-293 cells lysate 1200ug.
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Background
The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].
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