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Product Name
PDXK Polyclonal Antibody
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Description
Polyclonal antibody to PDXK
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PDXK antibody; C21orf124 antibody; C21orf97 antibody; HEL-S-1a antibody; PKH antibody; PNK antibody; PRED79 antibody; pyridoxal kinase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - PDXK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PDXK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Western blot - PDXK Polyclonal Antibody
Western blot analysis of extracts of various cells, using PDXK antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - PDXK Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PDXK antibody . Blue: DAPI for nuclear staining.
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Background
Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"