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Product Name
PDPK1 antibody
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Description
PDPK1 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 60-63 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hPDK1 antibody; PDK1 antibody; PDPK1 antibody; PRO0461 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDPK1 recombinant protein (Accession Number: NM_002613). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113635(PDPK1 antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using Catalog No:113635(PDPK1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PDPK1 represents 3-phosphoinositide dependent protein kinase-1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"