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Product Name
PDP1 Polyclonal Antibody
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Description
Polyclonal antibody to PDP1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PDP1 antibody; PDH antibody; PDP antibody; PDPC antibody; PPM2A antibody; PPM2C antibody; pyruvate dehyrogenase phosphatase catalytic subunit 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 278-537 of human PDP1 (NP_060914.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - PDP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PDP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - PDP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using PDP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PDP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spinal cord using PDP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PDP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using PDP1 antibody at dilution of 1:100 (40x lens).
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Background
Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"