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Product Name
PDLIM5 Polyclonal Antibody
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Description
Polyclonal antibody to PDLIM5
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
PDLIM5 antibody; ENH antibody; ENH1 antibody; L9 antibody; LIM antibody; PDZ and LIM domain 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 317-596 of human PDLIM5 (NP_006448.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PDLIM5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PDLIM5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - PDLIM5 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PDLIM5 antibody . Blue: DAPI for nuclear staining.
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Background
May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"