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Product Name
PDIA2 Polyclonal Antibody
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Description
Polyclonal antibody to PDIA2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PDIA2 antibody; PDA2 antibody; PDI antibody; PDIP antibody; PDIR antibody; protein disulfide-isomerase A2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 326-525 of human PDIA2 (NP_006840.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PDIA2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PDIA2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - PDIA2 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using PDIA2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"