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Product Name
PDHX antibody
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Description
PDHX Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells, HEK-293 cells, SKOV-3 cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DLDBP antibody; E3 binding protein antibody; E3BP antibody; OPDX antibody; PDHX antibody; PDX1 antibody; proX antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDHX recombinant protein (Accession Number: NM_003477). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IP: 1:2000-1:20000
IHC: 1:20-1:200
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113680(PDHX antibody) at dilution of 1:5000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113680(PDHX antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PDHX (IP:Catalog No:113680, 4ug; Detection:Catalog No:113680 1:5000) with HEK-293 cells lysate 2000ug.
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Background
PDHX represents pyruvate dehydrogenase complex, component X
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References
- Sol EM, Wagner SA, Weinert BT. Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3. PloS one. 7(12):e50545. 2012.
- Lehner C, Gehwolf R, Wagner A. Tendons from non-diabetic humans and rats harbor a population of insulin-producing, pancreatic beta cell-like cells. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme. 44(7):506-10. 2012.
- Li JT, Sun FX. Myocardin and pdx-1 synergistically induce hMSCs to differentiate into insulin secreting cells. Biochemical and biophysical research communications. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"