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Product Name
PDHB antibody
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Description
PDHB Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue. Positive IP detected in mouse heart tissue. Positive WB detected in human heart tissue, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp564K0164 antibody; PDHB antibody; PDHE1 B antibody; PHE1B antibody; Pyruvate Dehydrogenase E1 beta subunit antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDHB recombinant protein (Accession Number: NM_000925). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113679(PDHB antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:113679(PDHB antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:113679(PDHB antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PDHB (IP:Catalog No:113679, 4ug; Detection:Catalog No:113679 1:300) with mouse heart tissue lysate 4000ug.
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Background
PDHB(Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named as PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).
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References
- Thomas A, Stevens AP, Klein MS. Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet. Proteomics. 12(9):1437-51. 2012.
- Zhang Y, Pan YH, Yin Q. Critical roles of mitochondria in brain activities of torpid Myotis ricketti bats revealed by a proteomic approach. Journal of proteomics. 105:266-84. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"