-
Product Name
PDE5A antibody
- Documents
-
Description
PDE5A Rabbit Polyclonal antibody. Positive IP detected in SKOV-3 cells. Positive WB detected in SKOV-3 cells, NIH/3T3 cells. Positive IF detected in SKOV-3 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 100 kDa
-
Tested applications
ELISA, IF, WB, IP, IHC
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
CGB PDE antibody; CN5A antibody; PDE5 antibody; PDE5A antibody; PDE5A1 antibody; phosphodiesterase 5A antibody; cGMP antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PDE5A recombinant protein (Accession Number: NM_001083). Purification method: Antigen Affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:50-1:500
IF: 1:20-1:200
-
Validations
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113664(PDE5A Antibody) at dilution of 1:300
IP Result of anti-PDE5A (IP:Catalog No:113664, 4ug; Detection:Catalog No:113664 1:300) with SKOV-3 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:113664(PDE5A Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:113664(PDE5A Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of SKOV-3 cells using Catalog No:113664(PDE5A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
PDE5A (cGMP-binding cGMP-specific phosphodiesterase) plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Through alternative splicing, the PDE5A gene produces three proteins (PDE5A1, PDE5A2, and PDE5A3) that differ in their N termini and range in mass from 89 to 105 kDa. (PMID: 25704994; 21215707). A second 70 kDa band was consistently observed in heart tissue that either reflected a splice variant or proteolytic fragment of PDE5A. (PMID: 15576651).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"