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Product Name
PDE1B Polyclonal Antibody
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Description
Polyclonal antibody to PDE1B
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PDE1B antibody; HEL-S-79p antibody; PDE1B1 antibody; PDES1B antibody; phosphodiesterase 1B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-536 of human PDE1B (NP_000915.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - PDE1B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PDE1B antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - PDE1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using PDE1B antibody at dilution of 1:100 (40x lens).
Immunofluorescence - PDE1B Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PDE1B antibody . Blue: DAPI for nuclear staining.
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Background
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"