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Product Name
PDE1A antibody
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Description
PDE1A Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 61 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
61 kDa Cam PDE antibody; Cam PDE 1A antibody; hCam 1 antibody; HCAM1 antibody; HSPDE1A antibody; PDE1A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDE1A recombinant protein (Accession Number: XM_017004295). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113657(PDE1A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113657(PDE1A antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PDE1A (IP:Catalog No:113657, 4ug; Detection:Catalog No:113657 1:500) with mouse brain tissue lysate 4000ug.
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Background
PDE1A(Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)/calmodulin(PMID:12135876).
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