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PDCD4 antibody

Cat.#: 113765

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Product Information

  • Product Name
    PDCD4 antibody
  • Documents
  • Description
    PDCD4 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive FC detected in MCF-7 cells. Positive WB detected in HeLa cells, MCF7 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 54-64 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    H731 antibody; Nuclear antigen H731 like antibody; PDCD4 antibody; Protein 197/15a antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PDCD4 recombinant protein (Accession Number: NM_014456). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:1000-1:10000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113765(PDCD4 antibody) at dilution of 1:3000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113765(PDCD4 antibody) at dilution of 1:3000

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113765(PDCD4 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113765(PDCD4 antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-PDCD4 (IP:Catalog No:113765, 4ug; Detection:Catalog No:113765 1:3000) with HeLa cells lysate 4000ug.

    IP Result of anti-PDCD4 (IP:Catalog No:113765, 4ug; Detection:Catalog No:113765 1:3000) with HeLa cells lysate 4000ug.

    1X10^6 MCF-7 cells were stained with 0.2ug PDCD4 antibody (Catalog No:113765, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug PDCD4 antibody (Catalog No:113765, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.
  • References
    • Li J, Fu H, Xu C. miR-183 inhibits TGF-beta1-induced apoptosis by downregulation of PDCD4 expression in human hepatocellular carcinoma cells. BMC cancer. 10:354. 2010.
    • Hu JZ, Huang JH, Zeng L, Wang G, Cao M, Lu HB. Anti-apoptotic effect of microRNA-21 after contusion spinal cord injury in rats. Journal of neurotrauma. 30(15):1349-60. 2013.
    • Alayev A, Sun Y, Snyder RB, Berger SM, Yu JJ, Holz MK. Resveratrol prevents rapamycin-induced upregulation of autophagy and selectively induces apoptosis in TSC2-deficient cells. Cell cycle (Georgetown, Tex.). 13(3):371-82. 2014.
    • Alayev A, Berger SM, Kramer MY, Schwartz NS, Holz MK. The combination of rapamycin and resveratrol blocks autophagy and induces apoptosis in breast cancer cells. Journal of cellular biochemistry. 116(3):450-7. 2015.
    • Alayev A, Salamon RS, Sun Y. Effects of combining rapamycin and resveratrol on apoptosis and growth of TSC2-deficient xenograft tumors. American journal of respiratory cell and molecular biology. 53(5):637-46. 2015.
    • Zhen Y, Li D, Li W. Reduced PDCD4 Expression Promotes Cell Growth Through PI3K/Akt Signaling in Non-Small Cell Lung Cancer. Oncology research. 23(1):61-8. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"