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Product Name
PDAP1 antibody
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Description
PDAP1 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells. Positive IHC detected in human breast cancer. Positive IF detected in Hela. Observed molecular weight by Western-blot: 28kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
PDAP1 antibody; PAP antibody; PDGFA assOCiated protein 1 antibody; PAP1 antibody; PDGF assOCiated protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDAP1 recombinant protein (Accession Number: NM_014891). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113762(PDAP1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113762(PDAP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113762(PDAP1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using PDAP1 antibody Catalog No:113762 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PDAP1 represents PDGFA associated protein 1
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References
- Marimuthu A, Subbannayya Y, Sahasrabuddhe NA. SILAC-based quantitative proteomic analysis of gastric cancer secretome. Proteomics. Clinical applications. 7(5-6):355-66. 2013.
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