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PD-1/CD279 antibody

Cat.#: 113761

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Product Information

  • Product Name
    PD-1/CD279 antibody
  • Documents
  • Description
    PD-1/CD279 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, K-562 cells, mouse spleen tissue. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue, human ovary tumor tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 47 kDa
  • Tested applications
    ELISA, WB, FC, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CD279 antibody; hPD 1 antibody; hPD l antibody; PD 1 antibody; PD1 antibody; PD-1 antibody; PDCD1 antibody; programmed cell death 1 antibody; Protein PD 1 antibody; SLEB2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PD-1/CD279 recombinant protein (Accession Number: NM_005018). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113761(PD-1 antibody) at dilution of 1:500

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113761(PD-1 antibody) at dilution of 1:500

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:113761(PD-1/CD279 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:113761(PD-1/CD279 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:113761(PD-1/CD279 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:113761(PD-1/CD279 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    1X10^6 HeLa cells were stained with 0.2ug PD-1/CD279 antibody (Catalog No:113761, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug PD-1/CD279 antibody (Catalog No:113761, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438). Read more about this antibody on the blog: PD-1 blockade: Priming the Immune System to Combat Cancer
  • References
    • Jiang L, Zhao Z, Jiang S. Immunological markers predict the prognosis of patients with squamous non-small cell lung cancer. Immunologic research. 62(3):316-24. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"