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Product Name
PBRM1 antibody
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Description
PBRM1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in Transfected HEK-293 cells. Observed molecular weight by Western-blot: 180-195 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAF180 antibody; BRG1 assOCiated factor 180 antibody; hPB1 antibody; PB1 antibody; PBRM1 antibody; polybromo 1 antibody; Polybromo 1D antibody; Protein polybromo 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PBRM1 recombinant protein (Accession Number: BC015323). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113613(PBRM1 Antibody) at dilution of 1:1000
IP Result of anti-PBRM1 (IP:Catalog No:113613, 3ug; Detection:Catalog No:113613 1:300) with HEK-293 cells lysate 1800ug.
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Background
PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.
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