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Product Name
Paxillin antibody
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Description
Paxillin Rabbit Polyclonal antibody. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human liver cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, COLO 320 cells, Jurkat cells, MCF7 cells. Observed molecular weight by Western-blot: 68 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ16691 antibody; paxillin antibody; PXN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Recombinant Protein (Accession Number: NM_002859). Purification method: Protein A purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113611(PXN antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113611(PXN antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PXN (IP:Catalog No:113611, 3ug; Detection:Catalog No:113611 1:1000) with mouse brain tissue lysate 7500ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:113611(Paxillin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:113611(Paxillin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113611(PXN antibody) at dilution of 1:50 (under 10x lens)
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Background
PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).
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